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Table 1 Analysis of secretory granule content in corticotrophic and thyrotrophic cells before and after endotoxin administration

From: Localization of Macrophage Migration Inhibitory Factor (MIF) to Secretory Granules within the Corticotrophic and Thyrotrophic Cells of the Pituitary Gland

Granule Content

Time

0 hr

16 hr

Corticotrophes

  

MIF alone (%)

8.1 ± 3.9

4.9 ± 4.4 (p < 0.05)

MIF + ACTH (%)

24.4 ± 17.5

15.1 ± 5.8 (p = NS)

ACTH alone (%)

67.5 ± 18.3

80.0 ± 9.2 (p = NS)

Thyrotrophes

  

MIF alone (%)

20.1 ± 10.1

8.6 ± 4.4 (p < 0.05)

MIF + TSH (%)

13.7 ± 9.1

9.1 ± 5.0 (p = NS)

TSH alone (%)

66.3 ± 13.4

82.3 ± 8.2 (p = NS)

  1. Cells were stained with anti-MIF-anti-ACTH- and anti-TSH-labeled gold particles and quantified as described in the legend to Fig. 7 and Materials and Methods.