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Table 2 Total ACTH and MIF content in conditioned medium obtained 16 hr after stimulation of 1 × 10 6 AtT-20 cells with the indicated concentrations of CRH

From: Localization of Macrophage Migration Inhibitory Factor (MIF) to Secretory Granules within the Corticotrophic and Thyrotrophic Cells of the Pituitary Gland

CRH (ng/ml) ACTH (ng/ml) MIF (ng/ml)
1000 15.0 6.4
100 22.3 6.2
10 10.7 7.5
1 9.3 8.8
0.1 4.4 12.0
0.01 5.5
  1. ACTH was measured by a commercial radioimmunoassay. The MIF values were obtained by sandwich ELISA and are the mean of triplicate measurements.