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Table 2 Total ACTH and MIF content in conditioned medium obtained 16 hr after stimulation of 1 × 10 6 AtT-20 cells with the indicated concentrations of CRH

From: Localization of Macrophage Migration Inhibitory Factor (MIF) to Secretory Granules within the Corticotrophic and Thyrotrophic Cells of the Pituitary Gland

CRH (ng/ml)

ACTH (ng/ml)

MIF (ng/ml)

1000

15.0

6.4

100

22.3

6.2

10

10.7

7.5

1

9.3

8.8

0.1

4.4

12.0

0.01

5.5

  1. ACTH was measured by a commercial radioimmunoassay. The MIF values were obtained by sandwich ELISA and are the mean of triplicate measurements.