Effect of Ab to IL-6 on the disease course of EAE
(A) EAE was actively induced by immunizing SJL/J mice s.c. with MSCH in CFA and by giving pertussis toxin i.v. Two milligrams of the indicated mAb or the same volume of PBS was given i.p. on Day −1 and 7 (Table 1, Experiment 1). (B) EAE was adoptively transferred in PLSJLF1 mice by i.v. injection of 3.5–5 × 106 encephalitogenic T lymphocytes (reactive to MBPAc1-11). 0.5 mg of the anti-IL-6 Ab or the same volume of PBS was given i.p. on Day −1 or Day 6 (Table 2, Experiment 3). Animals were scored daily as described in Materials and Methods.