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Fig. 4 | Molecular Medicine

Fig. 4

From: Administration of Neutralizing Antibodies to Interleukin-6 (IL-6) Reduces Experimental Autoimmune Encephalomyelitis and Is Associated with Elevated Levels of IL-6 Bioactivity in Central Nervous System and Circulation

Fig. 4

Effect of neutralizing monoclonal Ab to IL-6 on IL-6 levels in serum and CSF

EAE was acitvely induced by immunizing SJL mice s.c. with MSCH in CFA and by giving pertussis toxin i.v. Animals received 1 mg of anti-IL-6 (20F3), anti-β-galactosidase (GL113) mAb, or the same volume of PBS i.p. on Day −1 or Day 8 relative to EAE induction. Animals were sampled 36 hr after EAE induction, at disease onset in the PBS-treated group, and 3 days after disease onset in the PBS-treated group. IL-6 levels in serum (A) and CSF (B) were determined by hybridoma growth factor assay. (n = 3/group; values are mean + SEM; nd, not done; detection limit in pooled CSF was 1.5 log10 U/ml)

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