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Table 2 Effect of antibodies to IL-6 on the disease course of adoptive transfer EAE

From: Administration of Neutralizing Antibodies to Interleukin-6 (IL-6) Reduces Experimental Autoimmune Encephalomyelitis and Is Associated with Elevated Levels of IL-6 Bioactivity in Central Nervous System and Circulation

Experimental Group Time of Treatment (days relative to transfer) Disease Incidence Mortality Mean Maximal Disease Score (±SEM) Mean Cumulative Disease Scorea
Absolute (±SEM) %
Experiment 1       
PBS −1, 4, 8 5/5 2/5 3.0 ± 0.84 28.6 ± 13.1 100
Anti-β-gal. −1, 4, 8 5/5 0/5 2.4 ± 0.25 20.8 ± 2.7 73
Anti-IL-6 −1, 4, 8 4/5 0/5 1.2 ± 0.37b 5.4 ± 2.6b 19
Experiment 2       
PBS −1, 4, 8 5/5 5/5 5.0 ± 0.00 68.6 ± 0.6 100
Anti-IL-6 −1, 4, 8 5/5 1/5c 3.4 ± 0.40b 44.2 ± 9.4 64
Experiment 3       
PBS −1, 6 10/10 6/10 4.2 ± 0.33 44.8 ± 4.2 100
Anti-β-gal. −1 5/5 3/5 4.2 ± 0.49 48.4 ± 8.1 108
  6 5/5 2/5 3.8 ± 0.49 46.0 ± 6.4 103
Anti-IL-6 −1 5/5 1/5 3.2 ± 0.49 32.4 ± 10.3 72
  6 5/5 1/5 2.8 ± 0.58b 32.2 ± 5.7 72
  1. EAE was adoptively transferred in PLSJLF1 mice by i.v. injection of 3.5–5 × 106 encephalitogenic T lymphocytes (reactive to MBPAc1-11). 0.5 mg of the mAb or the same volume of PBS were given i.p. on the indicated time points. Animals were scored daily as described in Materials and Methods.
  2. aThe mean cumulative disease score was calculated by adding the mean daily disease scores from all the animals in a group over the observation period (20 days) (expressed as the absolute value ± SEM and as the percentage of the score of the PBS group in each experiment). Scores for each group are compared with the scores for the PBS group in that experiment.
  3. bp < 0.05, Mann-Whitney U test.
  4. cp < 0.05, one-tailed Fisher exact test.