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Table 3 Rat IgG1 levels in serum and CSF after administration of rat IgG1 monoclonal Ab in animals with EAE

From: Administration of Neutralizing Antibodies to Interleukin-6 (IL-6) Reduces Experimental Autoimmune Encephalomyelitis and Is Associated with Elevated Levels of IL-6 Bioactivity in Central Nervous System and Circulation

Treatment Group

 

Rat IgG1 (µg/ml)

 

Seruma

CSFb

Noninduced

   

PBS

 

<1, <1, <1

<1

anti-IL-6

d−1

33, >40, 33

<1

 

d8

248, 254, 321 (274.3)

<1

anti-β-gal.

d−1

24, <1, <1

<1

 

d8

280, 242, 204 (242)

<1

EAE

   

PBS

 

<1, <1, <1

<1

anti-IL-6

d−1

1.1, 31, 5.2 (12.4)

1

 

d8

243, 310, 391 (314.7)

2.9

anti-β-gal.

d−1

<1, 2, 1

1

 

d8

216, 192, 200 (202.7)

3.4

  1. Rat IgG1 levels were determined by radial immunodiffusion on serum and CSF samples after administration of 1 mg of anti-IL-6 (20F3), anti-β-galactosidase (GL113), or PBS on either Day −1 (d−1) or Day 8 (d8) relative to active EAE induction. Animals were sampled at disease onset in the PBS group (Day 11–12).
  2. aSerum levels from individual mice are shown, the mean is given in parentheses where appropriate.
  3. bCSF levels are from pooled CSF (n = 3/group).
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Molecular Medicine

ISSN: 1528-3658