Contents of hsp70 and HO in human and rat islets prior to and after transplantation to nude mice
Precultured rat and human islets (5–8 days) were either frozen at −70°C or transplanted under the kidney capsule of nude mice (22). After 4 weeks in vivo, the transplanted islets were retrieved and used for immunoblot experiments. After incubation with anti-hsp70 antibodies, filters were reincubated with HO antibodies. Results are means ± SEM for three experiments. **p < 0.01 for a chance difference versus cultured rat islets and cultured human islets, respectively, using ANOVA and Scheffes F test. ***p < 0.001 versus cultured rat islets using ANOVA and Scheffes F test.