Effects of hydrogen peroxide, nicotinamide, and nitroprusside on mouse islet GSH contents
A suspension of dispersed mouse islet cells was incubated with the fluorescent probe monochlorobimane (40 µM) for 60 min at 37°C. After the preincubation period, the fluorescence was determined and set to 100% (initial value). Additions of (A) 0.1 and 0.2 mM hydrogen peroxide (HP) and (B) 50 mM nicotinamide or 0.2 mM sodium nitroprusside (NA and SP) were done directly after Time 0. The fluorescence was determined every 30 min for 180 min. Values are means ± SEM for three experiments.