Fig. 3
Time course of the induction of HO-1 mRNA in Hepa 1c1c7 cells
Cells were treated with the following inducers for 2, 4, 6, 8, and 10 hr: 100 µM tert-butylhydroquinone (1); 150 µM 1,2-dithiole-3-thione (2); 2.0 µM sodium arsenite (4); 100 µM trans-4-phenylbut-3-en-2-one (5); 100 µM 2,3-dimercapto-1-propanol (6); and 10 µM HgCl2 (7); 10 µM benzyl isothiocyanate (8). The mRNA was quantitated by Northern blot analysis, and the values were normalized to the 18S rRNA amounts on the same blots. Note the logarithmic scale of the relative RNA ratios.