Fig. 5
Response of the 9-kb upstream region of mouse HO-1 gene to inducers
Hepa 1c1c7 cells that had been stably transfected with the construct pMHO9cat, containing 9-kb of the 5′-upstream region of the HO-1 gene linked to the CAT reporter gene were treated with the following inducers: 25 µM tert-butylhydroquinone (Compound 1); 25 µM 1,2-dithiole-3-thione (Compound 2); 1.0 µM sodium arsenite (Compound 4); 50 µM trans-4-phenylbut-3-en-2-one (Compound 5); 12.5 µM 2,3-dimercapto-1-propanol (Compound 6); 2.5 µM HgCl2 (Compound 7); 2.5 µM benzyl isothiocyanate (Compound 8); and 100 nM phenylarsine oxide (Compound 9). Cells were harvested after 24 hr of treatment and CAT activity was determined. Results are presented as the relative CAT activities of treated to untreated cells, and were corrected for protein concentrations.