Fig. 6
Response of the SX2 upstream region of mouse HO-1 gene to inducers
Hepa 1c1c7 cells that had been stably transfected with the construct pMHOSX2cat, which contains the 268-bp SX2 fragment linked to the HO-1 minimal promoter, were treated with the following inducers: 25 µM tert-butylhydroquinone (Compound 1); 50 µM 1,2-dithiole-3-thione (Compound 2); 5 µM sulforaphane (Compound 3); 1.0 µM sodium arsenite (Compound 4); 50 µM trans-4-phenylbut-3-en-2-one (Compound 5); 30 µM 2,3-dimercapto-1-propanol (Compound 6); 3.0 µM HgCl2 (Compound 7); 2.5 µM benzyl isothiocyanate (Compound 8); 100 nM phenylarsine oxide (Compound 9); 2.5 µM 1-nitro-1-cyclohexene (Compound 10). Cells were harvested after 24 hr of treatment and CAT activities were determined. Results are presented as the relative CAT activities of treated to untreated cells, and were corrected for protein concentrations. Note the logarithmic scale of the relative CAT activities.