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Fig. 1 | Molecular Medicine

Fig. 1

From: Proteasome Inhibitors Prevent the Degradation of Familial Alzheimer’s Disease-Linked Presenilin 1 and Potentiate Aβ42 Recovery from Human Cells

Fig. 1

Wild-type and FAD-linked PS1 expression in stably transfected HEK293 cells. HEK293 cells were stably transfected with pcDNA3 containing wild-type PS1, ΔE9-PS1, or indicated missense mutated PS1. Transfected cells were grown in the presence of geneticin, then scraped, lysed in TBS-SDS, and 20 µg of protein was electrophoresed on a 12% SDS-PAGE analysis. PS1 holoproteins of high molecular weight (HMW-PS1), low molecular weight (LMW-PS1), and corresponding carboxyl-terminal fragment (CTF-PS1) were then revealed with αPS1Loop antibody as described in Materials and Methods.

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