Cell Line | Aβ42/Aβ total Ratio |
---|
Control | +Z-IE(Ot-Bu) A-Leucinal |
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Naive cells | 0.18 ± 0.007 | 0.16 ± 0.025 |
wtPS1-29 | 0.10 ± 0.001 | 0.13 ± 0.010 (100) |
M146V-PS1-8 | 0.24 ± 0.017 | 0.32 ± 0.015 (246) |
M146V-PS1-21 | 0.22 ± 0.020 | 0.27 ± 0.021 (208) |
H163R-PS1-9 | 0.25 ± 0.007 | 0.34 ± 0.040 (262) |
H163R-PS1-28 | 0.26 ± 0.030 | 0.41 ± 0.029 (315) |
E280A-PS1-12 | 0.24 ± 0.030 | 0.46 ± 0.032 (354) |
E280A-PS1-15 | 0.28 ± 0.024 | 0.45 ± 0.040 (346) |
ΔE9-PS1-45 | 0.23 ± 0.020 | 0.40 ± 0.032 (308) |
- Naive HEK293 cells or stable transfectants overexpressing wt, mutated, or ΔE9-PS1 were transiently transfected with wtβAPP751 cDNA. Thirty-six hours after transfection, cells were incubated for 16 hr in the absence (control) or presence of Z-IE(Ot-Bu)A-Leucinal (25 µM), then metabolically labeled for 6 hr in the presence of the inhibitor. Conditioned media were submitted to a subsequent two-step immunoprecipitation procedure with FCA3542 and FCA3340 antibodies (350-fold dilution). The ratios of Aβ42/Aβ total were estimated as in Figure 7. Values are the means ± S.E.M. of three to four independent experiments. Values in parentheses represent the Aβ42/Aβ total ratio expressed as the percent of that obtained with wtPS1-expressing cells (taken as 100).