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Table 2 Effect of Z-IE(Ot-Bu)A-Leucinal on A β secretion by independent clones expressing wild-type and FAD-linked PS1

From: Proteasome Inhibitors Prevent the Degradation of Familial Alzheimer’s Disease-Linked Presenilin 1 and Potentiate Aβ42 Recovery from Human Cells

Cell Line Aβ42/Aβ total Ratio
Control +Z-IE(Ot-Bu) A-Leucinal
Naive cells 0.18 ± 0.007 0.16 ± 0.025
wtPS1-29 0.10 ± 0.001 0.13 ± 0.010 (100)
M146V-PS1-8 0.24 ± 0.017 0.32 ± 0.015 (246)
M146V-PS1-21 0.22 ± 0.020 0.27 ± 0.021 (208)
H163R-PS1-9 0.25 ± 0.007 0.34 ± 0.040 (262)
H163R-PS1-28 0.26 ± 0.030 0.41 ± 0.029 (315)
E280A-PS1-12 0.24 ± 0.030 0.46 ± 0.032 (354)
E280A-PS1-15 0.28 ± 0.024 0.45 ± 0.040 (346)
ΔE9-PS1-45 0.23 ± 0.020 0.40 ± 0.032 (308)
  1. Naive HEK293 cells or stable transfectants overexpressing wt, mutated, or ΔE9-PS1 were transiently transfected with wtβAPP751 cDNA. Thirty-six hours after transfection, cells were incubated for 16 hr in the absence (control) or presence of Z-IE(Ot-Bu)A-Leucinal (25 µM), then metabolically labeled for 6 hr in the presence of the inhibitor. Conditioned media were submitted to a subsequent two-step immunoprecipitation procedure with FCA3542 and FCA3340 antibodies (350-fold dilution). The ratios of Aβ42/Aβ total were estimated as in Figure 7. Values are the means ± S.E.M. of three to four independent experiments. Values in parentheses represent the Aβ42/Aβ total ratio expressed as the percent of that obtained with wtPS1-expressing cells (taken as 100).