Fig. 1

PCR amplification of cDNAs containing BH1 and BH2 domains. Poly-A plus RNAs from AML cells with the indicated cytogenetic abnormalities were used as starting materials in RT-PCR reactions. The sequences of the primers were derived by synthesizing a family of oligonucleotides that contain the nucleic acid sequences of the BH1/BH2 domains. The predicted size of PCR products from correct priming is approximately 150 bp. Lane 1: MW marker (100 bp ladder). The cytogenetics of the cells in lanes 2–5 are monosomy 5, monosomy 7, inversion 16, and t(8;21), respectively. Plasmid DNA with bcl-2 insert served as the template in lane 6 as positive control.