Fig. 2
Expression of bcl-2 family members in AML cells. The composition of the BH1/BH2 amplification products was determined by cloning the PCR products followed by identification of the insert in each clone by slot-blot analysis of the bacterial colonies with probes specific to bcl-2, bc-xl, and bax.
The frequency of individual members was calculated by dividing the number of clones hybridized with a specific probe by the number of total clones containing the 150 bp insert. The abbreviations for cytogenetics are: −5, monosomy 5; −7, monosomy 7; inv 16, inversion 16; t(8;21), translocation 8;21. K562 cells were used as a control.