Ratio of bcl-xshort to bcl-xlong Is Different in Good- and Poor-Prognosis Subsets of Acute Myeloid Leukemia

Table 1 Southern blot analysis of PCR-amplified bcl-xlong and bcl-xshort products from AML cells

Patient	bclxl/bclxs	Cytogenetics	Patient	bclxl/bclxs	Cytogenetics
SN	5.0	-7	AE	1.9	t8;21
SS	2.7	+8	FY	1.8	+22,+8,inv16
HS	2.2	-7,inv3	BE	1.6	+8
SR	2.2	-9q,t8;21	MY	1.6	t8;21
HT	2.1	-5-7	EE	1.3	inv16
LE	1.9	-5-7	HE	1.3	inv16
LN	1.9	-5-7	TE	1.2	t8;21

RT-PCR was carried out with total RNA as templates and bcl-x specific oligonucleotides as primers. The PCR products were separated by agarose gel electrophoresis, transfered to nylon membrane in alkaline solution and hybridized with radioactive probes derived from bcl-x cDNA. The filter was exposed to multiple X-ray films. The ratio between the bcl-xlong signal and bcl-xshort signals was calculated from the image intensity on the films.

ISSN: 1528-3658