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Fig. 1 | Molecular Medicine

Fig. 1

From: Ursodeoxycholic Acid May Inhibit Deoxycholic Acid-Induced Apoptosis by Modulating Mitochondrial Transmembrane Potential and Reactive Oxygen Species Production

Fig. 1

MPT changes in isolated rat liver mitochondria incubated with bile acids. Mitochondria were isolated and incubated (1 mg protein/ml) with either DCA, UDCA, DCA + UDCA, or no bile acid (control) in respiration buffer as described in Materials and Methods. (A) Percent change in mitochondrial swelling was measured by monitoring the optical density at 540 nm. At time zero, 200 µM DCA was added and swelling was monitored for an additional 5 min. In the coincubation experiments, mitochondria were preincubated with 500 µM UDCA for 5 min. (B) Percent change in calcein release from calcein-loaded mitochondria was measured by monitoring the fluorescence using excitation and emission wavelengths of 490 and 515 nm, respectively. At time zero, 200 µM DCA was added and fluorescence was monitored for an additional 20 min. In the coincubation experiments, mitochondria were pretreated with 500 µM UDCA for 10 min. Values are mean ± standard deviations (S.D.) of at least five different experiments. *p < 0.001 from controls; §p < 0.001 from DCA.

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