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Fig. 4 | Molecular Medicine

Fig. 4

From: Ursodeoxycholic Acid May Inhibit Deoxycholic Acid-Induced Apoptosis by Modulating Mitochondrial Transmembrane Potential and Reactive Oxygen Species Production

Fig. 4

Reduction of ΔΨm and increased production of ROS after incubation of isolated mitochondria with PhAsO. Isolated mitochondria were incubated with 80 µM PhAsO, 500 µM UDCA, 80 µM PhAsO + 500 µM UDCA, or no addition (control) for 5 min. In the coincubation experiments, mitochondria were pretreated with UDCA alone for 5 min prior to addition of PhAsO. Isolated mitochondria (1 mg protein/ml) were suspended in respiration buffer and incubated for 15 min at 37°C with 50 nM DiOC6(3), 2 µM HE, or 5 µM H2DCFDA and analyzed by cytofluorometry. The percentages reflect (A) the disruption in ΔΨm; (B) the increased production of superoxides; and (C) the increased production of peroxides during treatment with PhAsO, and the respective inhibition by UDCA. The treatment groups are indicated on the left and the data shown are representative of at least three different experiments. Coincubation with UDCA was associated with significant inhibition of mitochondrial perturbation (p < 0.05, or lower).

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