Fig. 6
Western blot analysis of apoptosis-associated proteins in liver from bile acid-fed rats. Cytoplasmic proteins (150 µg/lane) from control, DCA, UDCA, and DCA + UDCA fed rats were isolated from whole liver as described in Materials and Methods. Following SDS-PAGE and transfer, the nitrocellulose membranes were incubated with antibodies to either Bax, Bad, Bcl-2, or Bcl-XL and the proteins were detected using ECL chemiluminescence. Representative Western blots of cytoplasmic proteins are shown at the top and the accompanying histograms below depict the mean changes ± standard error of the mean (S.E.M.) in protein levels relative to controls. The proteins are indicated on the left and the values shown are from at least three different animals from each group. §p < 0.001 from Bad control; p < 0.05 from Bcl-XL control.