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Fig. 7 | Molecular Medicine

Fig. 7

From: Ursodeoxycholic Acid May Inhibit Deoxycholic Acid-Induced Apoptosis by Modulating Mitochondrial Transmembrane Potential and Reactive Oxygen Species Production

Fig. 7

Western blot analysis of apoptosis-associated proteins in mitochondria isolated from livers of bile acid-fed rats. Mitochondrial proteins (150 µg/lane) from control, DCA, UDCA, and DCA + UDCA fed rats were isolated from whole liver as described in Materials and Methods. Following SDS-PAGE and transfer, the nitrocellulose membranes were incubated with antibodies to either Bax, Bad, Bcl-2, or Bcl-XL and the proteins were detected using ECL chemiluminescence. Representative Western blots of mitochondrial proteins are shown at the top and the accompanying histograms depict the mean changes ± S.E.M. in protein levels relative to control. The proteins are indicated on the left and the values shown are from four different animals from each group. *p < 0.001 from control; §p < 0.05 from control.

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