Fig. 3
NO inhibits viral replication and protein biosynthesis in primary mouse peritoneal macrophages. (A) Peritoneal macrophages from iNOS knock-out and wild-type mice were stimulated with LPS + IFN-γ for 24 hr and \({\rm{NO}}_2^ - \) levels determined in the culture media by the Griess reaction. (B) After 8 hr stimulation with LPS + IFN-γ ± NMA, peritoneal macrophages were infected with vaccinia virus. After an 18 hr incubation, viral proliferation was measured by plaque-forming assay. Protein synthesis was assessed by measuring 35S-methionine incorporation into proteins after a 4 hr labeling interval. All data are expressed as mean ± SD of two independent experiments, each performed in triplicate.