Fig. 7
From: Lasp-1, a Novel Type of Actin-Binding Protein Accumulating in Cell Membrane Extensions
Tyrosine phosphorylation of Lasp-1 in 3T3/A cells transformed with the c-SrcY527F variant. Crude extracts from 3T3/N (lane 1) and 3T3/A (lane 2), and Lasp-1 immunopurified with the pab805 antibody from 3T3 (lane 3), 3T3/N (lane 4), and 3T3/A (lane 5) were analyzed using the 4G10 antiphosphotyrosine (anti PY) antibody (upper panel). The same blot was stripped and probed with the pab805 antibody (lower panel).