Expression of the pNcN transgene in transgenic mice. (A) RT-PCR was performed on ovary, lung, heart, liver and kidney of a virgin female and testis of a male NN60 transgenic mouse using SV40 polyA primers. (B) RT-PCR with SV40 polyA primers used on female mammary gland tissue from virgin 7-day pregnant, lactating and 9-day regressed transgenic mice. A wild type virgin is also shown for control of primer specificity. These samples underwent 40 rounds of PCR amplification.