Fig. 9
From: Activator Protein-1 Mediates Induced but not Basal Epidermal Growth Factor Receptor Gene Expression
Endogenous EGFR is increased in MCF-7 cells over-expressing c-Jun. Stable transfectants of MCF-7 expressing neomycin resistance (7-1 and 7-2) or c-jun (2–16, 2–33 and 2–31) were grown in selective media and protein extracts prepared. Extracts from MCF-7 and WI-38 cells were prepared in an identical fashion. 50 µg of each extract was fractionated on polyacrylamide gels and subjected to western blotting using EGFR and actin antibodies.