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Fig. 3 | Molecular Medicine

Fig. 3

From: Activation of Platelet-activating Factor (PAF) Receptor Stimulates Nitric Oxide (NO) Release Via Protein Kinase C-α in HEC-1B Human Endometrial Epithelial Cell Line

Fig. 3

Dose-dependent release of nitric oxide (NO) from human HEC-1B cells. HEC-1B cells were plated at a density of 300,000 cells/ml in 24 well plates and grown to 80% confluency in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal calf serum. Following serum starvation, near confluent monolayers were stimulated for 30 min with an increasing concentration of platelet-activating factor (PAF, solid circle) or an increasing concentration of platelet-activating factor in the presence of 10 µM WEB2170 (open circle). The media was removed and NO levels determined using a Sievers NOA 270B chemiluminescent analyser. The results are expressed as means ± S.E.M. of a typical experiment (triplicate determinations per experiment; 3 similar experiments). Basal NO release was 1482 pmol/well and has not been subtracted from the data.

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