Fig. 2
From: Myocardial Postischemic Injury Is Reduced by PolyADPribose Polymerase-1 Gene Disruption
Immunohistochemical staining for polyADPribose (PAR).
Control hearts were perfused for 40 min with normal saline and experimental hearts underwent 30 min of global ischemia and 10 min of reperfusion. Triplicate hearts were studied for each condition with representative results shown. PAR staining is increased dramatically following I/R in WT heart. Basal PAR staining in PARP-1−/− heart is substantially less than in WT heart. Staining for PAR in PARP-1−/− heart increases following I/R to levels that appear comparable to basal PAR staining seen in WT heart. WT = wild type; PARP-1−/− = polyADPribose polymerase-1 knockout; I/R = ischemia-reperfusion.