Fig. 4

Tolerization normalizes the T-cell repertoire.

Estimate of the Vβ usage in phosphate-buffered saline (PBS) treated and tolerized mice. Squares represent an expansion of clones, while circles represent deletion of clones in either the spleen or lymph nodes. The colors of the symbols stand for a semi-quantitative assessment of the modification and was determined by two independent observers. The white symbols are for a moderate modification, the black for an extended modification, and the grey for intermediate modification. For an example of the length analysis profile, see Figure 3. The Vβ12 was presenting an atypical length analysis profile and was analyzed separately (Figure 3).