Fig. 10
Association of villin with the p85 subunit of PI-3 kinase.
Villin (A), PLC-γ1 (B) and p85 (D) was immunoprecipitated from the TritonX-100-soluble and insoluble fractions of control (−) and Na+/Pi cotransport up-regulated cells (+). In different cell extracts, PLC-γ1 was immunodepleted and the remaining soluble and insoluble fractions were reprecipitated with anti-villin anti-body (C). Nitrocellulose membranes were probed with monoclonal antibodies against phosphotyrosine (A) and p85 (B). Std: positive control for p85 (representative of a total of 3 experiments). PI-3 kinase, phosphatidylinositol-3 kinase; p85, the 85 KDa subunit of PI-3 kinase; P-Tyr, phosphotyrosine.