Fig. 4
Binding of villin to actin simultaneously with their redistribution from the TritonX-100-insoluble to soluble cytoskeletal fraction, during Na+/Pi cotransport upregulation.
Villin was immunoprecipitated from the TritonX-100-soluble and insoluble fractions of control (−) and Na+/Pi cotransport up-regulated cells (+). Nitrocellulose membranes were probed with monoclonal antibodies against villin (A) and actin (B) (representative of a total of 4 experiments). Ipt, immunoprecipitation; Blot, Western Blot Analysis.