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Fig. 4 | Molecular Medicine

Fig. 4

From: PLC-γ1 Signaling Pathway and Villin Activation Are Involved In Actin Cytoskeleton Reorganization Induced by Na+/Pi Cotransport Up-regulation

Fig. 4

Binding of villin to actin simultaneously with their redistribution from the TritonX-100-insoluble to soluble cytoskeletal fraction, during Na+/Pi cotransport upregulation.

Villin was immunoprecipitated from the TritonX-100-soluble and insoluble fractions of control (−) and Na+/Pi cotransport up-regulated cells (+). Nitrocellulose membranes were probed with monoclonal antibodies against villin (A) and actin (B) (representative of a total of 4 experiments). Ipt, immunoprecipitation; Blot, Western Blot Analysis.

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