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Fig. 5 | Molecular Medicine

Fig. 5

From: PLC-γ1 Signaling Pathway and Villin Activation Are Involved In Actin Cytoskeleton Reorganization Induced by Na+/Pi Cotransport Up-regulation

Fig. 5

PLC-γ 1 translocation to the actin cytoskeleton.

PLC-γ1 was immunoprecipitated from the TritonX-100-soluble and insoluble fractions of control (−) and Na+/Pi cotransport up-regulated cells (+). Nitrocellulose membrane was probed with monoclonal antibodies against phosphotyrosine (A), PLC-γ1 (B) and actin (C). Std: positive control for PLC-γ1 (representative of a total of 8 experiments). PLC-γ1, phospholipase Cγ1; P-Tyr, phosphotyrosine; IgG(H), Immunoglobulin G (Heavy chain); Blot, Western Blot Analysis.

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