PLC-γ 1 translocation to the actin cytoskeleton.
PLC-γ1 was immunoprecipitated from the TritonX-100-soluble and insoluble fractions of control (−) and Na+/Pi cotransport up-regulated cells (+). Nitrocellulose membrane was probed with monoclonal antibodies against phosphotyrosine (A), PLC-γ1 (B) and actin (C). Std: positive control for PLC-γ1 (representative of a total of 8 experiments). PLC-γ1, phospholipase Cγ1; P-Tyr, phosphotyrosine; IgG(H), Immunoglobulin G (Heavy chain); Blot, Western Blot Analysis.