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Fig. 6 | Molecular Medicine

Fig. 6

From: PLC-γ1 Signaling Pathway and Villin Activation Are Involved In Actin Cytoskeleton Reorganization Induced by Na+/Pi Cotransport Up-regulation

Fig. 6

A tyrosine phosphorylated protein mediates the association of PLC-γ1 with actin.

Tyrosine phosphorylated proteins were immunoprecipitated from the TritonX-100-soluble and insoluble fractions of control (−) and Na+/Pi co-transport up-regulated cells (+). Nitrocellulose membrane was probed with monoclonal antibodies against actin (A) and phosphotyrosine (B). In different cell extracts, PLC-γ1 was immunodepleted and the remaining soluble and insoluble fractions were reprecipitated with anti-phosphotyrosine antibody. Nitrocellulose membrane was probed with monoclonal antibody against actin (C) (representative of a total of 6 experiments). PLC-γ1, phospholipase Cγ1; P-Tyr, phosphotyrosine; IgG(H), Immunoglobulin G (H); Blot, Western Blot Analysis.

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