A tyrosine phosphorylated protein mediates the association of PLC-γ1 with actin.
Tyrosine phosphorylated proteins were immunoprecipitated from the TritonX-100-soluble and insoluble fractions of control (−) and Na+/Pi co-transport up-regulated cells (+). Nitrocellulose membrane was probed with monoclonal antibodies against actin (A) and phosphotyrosine (B). In different cell extracts, PLC-γ1 was immunodepleted and the remaining soluble and insoluble fractions were reprecipitated with anti-phosphotyrosine antibody. Nitrocellulose membrane was probed with monoclonal antibody against actin (C) (representative of a total of 6 experiments). PLC-γ1, phospholipase Cγ1; P-Tyr, phosphotyrosine; IgG(H), Immunoglobulin G (H); Blot, Western Blot Analysis.