The inhibition of tyrosine phosphorylation abolishes PLC-γ1 translocation to actin cytoskeleton, as well as its association with villin.
Control (−) and Na+/Pi cotransport up-regulated cells (+) were preincubated with genistein (100 µM, 40 min) or with its vehicle (dimethylsulfoxide; DMSO). PLC-γ1 was immunoprecipitated from equal volumes of TritonX-100-soluble and insoluble fractions. Nitrocellulose membranes of untreated and genistein treated cells were probed with monoclonal anti-PLC-γ1 (A and B) and anti-villin (C and D) antibody (representative of a total of 2 experiments). PLC-γ1, phospholipase Cγ1; P-Tyr, phosphotyrosine.