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Fig. 9 | Molecular Medicine

Fig. 9

From: PLC-γ1 Signaling Pathway and Villin Activation Are Involved In Actin Cytoskeleton Reorganization Induced by Na+/Pi Cotransport Up-regulation

Fig. 9

The inhibition of tyrosine phosphorylation abolishes PLC-γ1 translocation to actin cytoskeleton, as well as its association with villin.

Control (−) and Na+/Pi cotransport up-regulated cells (+) were preincubated with genistein (100 µM, 40 min) or with its vehicle (dimethylsulfoxide; DMSO). PLC-γ1 was immunoprecipitated from equal volumes of TritonX-100-soluble and insoluble fractions. Nitrocellulose membranes of untreated and genistein treated cells were probed with monoclonal anti-PLC-γ1 (A and B) and anti-villin (C and D) antibody (representative of a total of 2 experiments). PLC-γ1, phospholipase Cγ1; P-Tyr, phosphotyrosine.

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