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Table 2 ACE kinetic constants (Km and Vmax) determination in plasma and homogenized lung using specific C- and N-domain substrates.

From: Exclusion of Angiotensin I-Converting Enzyme as a Candidate Gene Involved In Exudative Inflammatory Resistance in F344/N Rats

  

C-domain Substrate (HHL)

N-domain Substrate (AcSDAcKP)

  

Km (mM)

Vmax (nmol/ml/min)

Km (mM)

Vmax (nmol/ml/min)

Plasma

LEW/N

2.25 ± 0.07

3.63 ± 0.06

2.12 ± 0.82

0.14 ± 0.03

 

F344/N

2.04 ± 0.11

3.41 ± 0.09

2.47 ± 0.88

0.21 ± 0.04

Lung

LEW/N

1.32 ± 0.13

5.09 ± 0.21

7.36 ± 1.96

0.63 ± 0.14

 

F344/N

1.41 ± 0.13

5.04 ± 0.20

5.65 ± 1.94

0.33 ± 0.09

  1. Each Km and Vmax value ± S.E. was computed by the ENZFITTER program from iteration on 24 individual points. Pooled samples (n = 8) were used in these experiments. See “Materials and Methods” for more details.
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Molecular Medicine

ISSN: 1528-3658