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Table 2 ACE kinetic constants (Km and Vmax) determination in plasma and homogenized lung using specific C- and N-domain substrates.

From: Exclusion of Angiotensin I-Converting Enzyme as a Candidate Gene Involved In Exudative Inflammatory Resistance in F344/N Rats

   C-domain Substrate (HHL) N-domain Substrate (AcSDAcKP)
   Km (mM) Vmax (nmol/ml/min) Km (mM) Vmax (nmol/ml/min)
Plasma LEW/N 2.25 ± 0.07 3.63 ± 0.06 2.12 ± 0.82 0.14 ± 0.03
  F344/N 2.04 ± 0.11 3.41 ± 0.09 2.47 ± 0.88 0.21 ± 0.04
Lung LEW/N 1.32 ± 0.13 5.09 ± 0.21 7.36 ± 1.96 0.63 ± 0.14
  F344/N 1.41 ± 0.13 5.04 ± 0.20 5.65 ± 1.94 0.33 ± 0.09
  1. Each Km and Vmax value ± S.E. was computed by the ENZFITTER program from iteration on 24 individual points. Pooled samples (n = 8) were used in these experiments. See “Materials and Methods” for more details.