Fig. 1

β-cell differentiation in the presence of laminin-1 (200 µg/ml) and rat GoH3 blocking monoclonal antibody to α₆ integrin. (A) Fetal mouse pancreas cells (13.5 dpc) cultured for 4 days with (1) and without (2) GoH3 (40 µg/ml), under phase contrast microscopy (top panel) and after staining with guinea pig anti-insulin serum and peroxidase (lower panel). (B) Numbers of β cells and total cells after culture of 13.5-dpc fetal mouse pancreas cells for 4 days with GoH3 (open bar) or rat IgG2a isotype control antibody (solid bar). *p, 0.05, **p, 0.01 versus in the absence of GoH3 or in the presence of isotope control antibody. (C) Number of β cells^{^} (open bar) and total cells (solid bar) after culture of 13.5-dpc fetal mouse pancreas cells for 4 days with GoH3 (40 µg/ml) in the presence or absence of laminin-1. *p, 0.001 versus in the presence of both laminin-1 and GoH3. **p, 0.01 versus in the presence of laminin-1 alone. Cell counts, as described in “Materials and Methods,” were expressed as mean 6 SEM (n 5 3 separate experiments).