Fig. 2

Number of β cells (open bar) and total cells (solid bar) in the presence of PI3K inhibitor wortmannin (A) or Ly294002 (B), F-actin polymerization inhibitor cytochalasin D (C), MEK1 inhibitor PD98059 (D), and Src family tyrosine kinase inhibitors genistein (E) and herbimycin A (F). β Cells were identified by staining with guinea pig anti-insulin serum and peroxidase and cell counts were expressed as mean 6 SEM (n 5 3 separate experiments). The left panels show number of β cells and total cells in the presence of laminin-1 (200 µg/ml) and the indicated concentrations of inhibitors. *p, 0.05, **p, 0.01 versus in the absence of wortmannin, Ly 294002, cytochalasin D or PD 98059. The right panels show number of β cells and total cells in the presence or absence of laminin-1 (200 µg/ml) and wortmannin (10 µM), Ly294002 (100 µM), or cytochalasin D (10 µM). *p, 0.001 versus in the presence of laminin-1 and wortmannin or Ly294002. **p, 0.01 versus in the presence of laminin-1 alone.