Fig. 4From: Functional Characterization of Three Mutations of the Endothelin B Receptor Gene in Patients With Hirschsprung’s Disease: Evidence for Selective Loss of Gi CouplingMeasurement of ET-1-induced calcium transients in CHO cells bearing WT and mutant hETB receptors. CHO cells producing æquorin (27) were transiently transfected with cDNA encoding WT (d), or mutant G57S (□), R319W (△) and P383L (◇) hETB receptors. Light emission was measured immediately after stimulation with various concentrations of ET-1 in presence of cœlenterazine. The light emission measured is representative of the calcium transients. Results are expressed in counts per second (cps) and represent the means 6 SEM of three independent experiments.Back to article page