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Fig. 2 | Molecular Medicine

Fig. 2

From: α 1 Na,K-ATPase and Na,K,2Cl-Cotransporter/D3mit3 Loci Interact to Increase Susceptibility to Salt-Sensitive Hypertension in Dahl SHSD Rats

Fig. 2

Differential expression of Na,K,2Cl-cotransporter isoforms, NKC-B, NKC-A, and NKC-F in 10 week-old Dahl S and Dahl R kidneys. (a) Densitometric quantitative analysis of RT-PCR products detect equivalent amounts of total NKC mRNA levels in 10-week-old male Dahl S (ST) and Dahl R (RT) kidneys. Data shown are the mean values (±SEM) of 6 Dahl S and 6 Dahl R kidneys (p = 0.77, one-way ANOVA). β-actin RT-PCR products from Dahl S (SβA) and Dahl R (RβA) are shown as controls. (b) Densitometric quantitative analysis of RT-PCR products detect different levels of each NKC isoform, NKC-B, NKC-A, and NKC-F, between 10-week-old male Dahl S and Dahl R kidneys resulting in differential isoform ratios. Data shown are the mean values (± SEM) of six Dahl S and six Dahl R kidneys. One-way ANOVA statistical analysis *p = 0.02; **p < 0.0001; ***p < 0.0001. Different amounts of total kidney RNA samples were used as noted (Sample [µg]) to obtain RT-PCR amplified product at exponential phase (see Materials and Methods for details).

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