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Fig. 3 | Molecular Medicine

Fig. 3

From: Role of Transcription Factor Nrf2 in the Induction of Hepatic Phase 2 and Antioxidative Enzymes in vivo by the Cancer Chemoprotective Agent, 3H-1, 2-Dithiole-3-thione

Fig. 3

Hepatic Nrf2 protein levels after treatment with D3T. Hepatic nuclear extract (A), cytosolic, and total homogenate fractions (B) were prepared from livers of wild-type mice and electrophoresed on a 6% acrylamide gel. Immunoblot analysis was carried out using Nrf2 antibody reacting with the N-terminal of Nrf2. Each sample was prepared from four pooled livers at 2, 6, and 24 hr after treatment with D3T (0.5 mmol/kg p.o.) or 6 hr after vehicle treatment. Three similar immunoblots were obtained.

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