Role of Transcription Factor Nrf2 in the Induction of Hepatic Phase 2 and Antioxidative Enzymes in vivo by the Cancer Chemoprotective Agent, 3H-1, 2-Dithiole-3-thione

Table 1 Oligonucleotide sequences for PCR amplification of murine phase 2 and antioxidative enzymes and probe sequences of NFE2, mouse GST Ya ARE, and human NQO1 ARE

Enzymes		Primers	Gene Bank Accession No
Nrf2	Sense	ATGGATTTGATTGACATCCTT	U20532
	Antisense	CATGTTTTTCTTTGTATCTGG
NQO1	Sense	ATCCTTCCGAGTCATCTCTA	U12961
	Antisense	CAACGAATCTTGAATGGAGG
HO-1	Sense	AGGTGTCCAGAGAAGGCTT	X13356
	Antisense	ATCTTGCACCAGGCTAGCA
yGCSr	Sense	AGGAGCTTCGGGACTGTATT	U95053
	Antisense	TGGGCTTCAATGTCAGGGAT
UGT1A6	Sense	TGATGCTCTGTTCACAGACC	U09930
	Antisense	AATGCCCGAGTATTTGGATG
AFAR	Sense	TGCCTCAGACACTTCGGA	AW108455
	Antisense	TAGTTGGGACACTCGTGG
MnSOD	Sense	TGAACAACCTCAACGCCAC	Z18857
	Antisense	GAAGGTAGTAAGCGTGCTC
Catalase	Sense	AACTGGGATCTTGTGGGAA	L25069
	Antisense	GACAGTTCACAGGTATCTG
Albumin	Sense	TCCATGTATGAAAAGTGCTG	AJ011080
	Antisense	CTGCAGCAGTCTTCTAAGAA
NFE2		TGGGGAACCTGTGCTGAGTCACTGGT
mGST Ya ARE		AGCTTGGAAATGACATTGCTAATGGTGACAA
		AGCAACTTTGTCGAC
hNQO1 ARE		GCAGTCACAGTGACTCAGCAGAATCT

ISSN: 1528-3658