Nrf2 Genotype | +/+ | −/− | +/+ | −/− |
---|
Enzyme | Constitutive Levels | Inducible Levels |
---|
GST Ya | 1 | 0.63 | 6.38a | 0.79 |
GST Yp | 1 | 0.28 | 4.93a | 0.66b |
GST Yb | 1 | 1.26 | 2.63a | 0.87 |
GST Yc | 1 | 0.75 | 1.07 | 0.29b |
NQO1 | 1 | 1.11 | 5.95a | 0.67 |
UGT1A6 |
1
| 0.99 | 3.63a | 0.24b |
mEH | 1 | 0.52 | 4.02a | 1.13 |
γGCSr |
1
| 1.29 | 2.84a | 1.40 |
AFAR | 1 | 0.73 | 1.13 | 0.15b |
HO-1 |
1
| 1.66 | 5.89a | 0.72 |
Ferritin Light |
1
| 0.94 | 1.53 | 2.45b |
Ferritin Heavy | 1 | 0.58 | 1.13 | 2.08b |
MnSOD | 1 | 1.31 | 2.02a | 0.05b |
Catalase |
1
| 1.08 | 1.40 | 0.39b |
- Relative mRNA levels were analyzed after D3T treatment in nrf2 wild-type and mutant type mouse liver. GST Ya, Yp, Yb, Yc, mEH, FH, and FL mRNA were detected using northern blot hybridization and NQO1, UGT1A6, γGCSr, HO-1, AFAR, MnSOD, and CAT levels were detected using RT-PCR. Values are the means of determinations done on three mice per genotype. Levels of RNA for each gene were normliazed to albumin mRNA levels and expressed as a ratio over vehicle-treated wild-type control. Transcript levels were measured 24 hr after treatment with D3T for all genes except HO-1, which was measured at 6 hr.
- ap, 0.05, compared to vehicle-treated 2/1.
- bp, 0.05, compared to vehicle-treated 2/2 group.