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Fig. 5 | Molecular Medicine

Fig. 5

From: Interleukin-6 and Glucocorticoids Synergistically Induce Human Immunodeficiency Virus Type-1 Expression in Chronically Infected U1 Cells by a Long Terminal Repeat Independent Post-Transcriptional Mechanism

Fig. 5

LTR-independent synergistic induction of HIV expression in U1 cells by IL-6 and Dex. (A) Activation of supernatant-associated RT and CAT activities in U1 cells transiently transfected with an HIV-1 LTR-CAT construct and stimulated with different concentrations of TNF-α 48 h after transfection. Although quantitatively different, the sensitivity of U1 cells to the activating effect of TNF-α was comparable in both assays. (B) Lack of HIV LTR activation by stimulation of U1 cells with IL-6, in the presence or absence of Dex. The results represent the mean ± SD of 4 independent experiments. In no experiments did IL-6 alone, Dex alone, or their combination induce CAT activity at levels above 2-fold the low constitutive levels observed in unstimulated U1 cells.

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