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Table 1 Specific binding of GC in U1 cells

From: Interleukin-6 and Glucocorticoids Synergistically Induce Human Immunodeficiency Virus Type-1 Expression in Chronically Infected U1 Cells by a Long Terminal Repeat Independent Post-Transcriptional Mechanism

Total Binding (cpm)

Nonspecific Binding (cpm)

Specific Binding (cpm)

Receptors Per Cell

1,081 ± 28

424 ± 15

657 ± 17

3,800 ± 98

  1. Duplicate samples of 5 × 106 U1 cells were labeled in the presence of [3H]-Dex (total binding) or [3H]-Dex plus an excess of unlabeled steroid (nonspecific binding). Specific binding to GC receptors was determined by subtracting the nonspecific binding value from that of the total binding, whereas the number of receptors per cell (± S.E.M.) was determined from the specific activity of the labeled Dex.
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Molecular Medicine

ISSN: 1528-3658