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Table 1 Infectivity of steel or gold wires after exposure to intact brain or to brain homogenate of scrapie-infected mice

From: Transmission of Scrapie by Steel-surface-bound Prions

Inoculation Sick/Total Incubation Time ± s.d. (days)
Experiment 1   
Wire transiently inserted for 5 min 5/5 68 ± 2
for 30 min 6/6 65 ± 4
for 120 min 6/6 69 ± 5
Wire exposed to 10% brain homogenate+ 7/7 75± 5
Brain homogenate+ (1%, 0.03 ml) 4/4 68± 8
Experiment 2   
Wires exposed to homogenate   
Steel wire (10%, w/v) 4/4 85± 4
Gold wire (10%, w/v) 3/3 74± 2
Steel wire (1%, w/v) 4/4 86± 8
Gold wire (1%, w/v) 4/4 81± 6
  1. For experiment 1, two C57BL/6 mice were culled 87 days after i.c. inoculation with RML, that is, about 2 months before appearance of clinical symptoms. Wires were inserted into brain for the time indicated or exposed to centrifuged 10% brain homogenate for 16 h and processed as described in the Methods section. For experiment 2, wire segments were exposed to centrifuged brain homogenate of RML-infected, terminally sick CD1 mice as described in Methods.
  2. +6.8 logLD50 units/ml 10% homogenate, as determined by end point titration (23) in Tga20 mice.