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Table 4 Infectivity of surface-bound mouse prions after various treatments

From: Transmission of Scrapie by Steel-surface-bound Prions

Inoculation Sick/Total Incubation Time ± s.d. (days)
1. Uninfected wires   
Untreated 0/3 >260
2. Infectious wires   
Untreated 6/6 76 ± 5
Sodium hydroxide (1M, 1 h, 25°C) 0/6 >260
Formaldehyde (10%, 1 h, 25°C) 6/6 92 ± 8
Guanidinium thiocyanate (4M, 16 h, 25°C) 0/6 >260
  1. Infectious wires were prepared with centrifuged brain homogenates and assayed as described (11). End point titration (23) of the homogenate gave a titre of 6.75 log LD50 units/ml 10% homogenate. NaOH and formaldehyde solutions were prepared immediately prior to use; 4 M guanidinium thiocyanate was RNA Lysis buffer (#40082, Applied Biosystems, Foster City, CA, USA). Wires were exposed to 1 ml solution and washed with 1 ml PBS four times prior to implantation.