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Table 4 Infectivity of surface-bound mouse prions after various treatments

From: Transmission of Scrapie by Steel-surface-bound Prions

Inoculation

Sick/Total

Incubation Time ± s.d. (days)

1. Uninfected wires

  

Untreated

0/3

>260

2. Infectious wires

  

Untreated

6/6

76 ± 5

Sodium hydroxide (1M, 1 h, 25°C)

0/6

>260

Formaldehyde (10%, 1 h, 25°C)

6/6

92 ± 8

Guanidinium thiocyanate (4M, 16 h, 25°C)

0/6

>260

  1. Infectious wires were prepared with centrifuged brain homogenates and assayed as described (11). End point titration (23) of the homogenate gave a titre of 6.75 log LD50 units/ml 10% homogenate. NaOH and formaldehyde solutions were prepared immediately prior to use; 4 M guanidinium thiocyanate was RNA Lysis buffer (#40082, Applied Biosystems, Foster City, CA, USA). Wires were exposed to 1 ml solution and washed with 1 ml PBS four times prior to implantation.