Fig. 2

Vγ9Vδ2 cytotoxicity toward NHL OCI-Ly8. (A) Effect of antagonist anti-Fas ZB4 antibody on Vγ9Vδ2 T-cell cytotoxicity toward Daudi, OCI-Ly8, and Jurkat cells in a 4-hr ⁵¹chromium release assay. Antagonist effect of ZB4 is demonstrated by its ability to inhibit Fas⁺-Jurkat cells lysis induced in the same conditions by the Fas agonist CH11 mAb. Isotype-matched IgG1 antibody has no effect on CH11-induced lysis of Jurkat cells (not shown). ZB4 mAb concentrations are shown in insert box. NT, not tested; E/T = 20/1. (B) Effect of Ca²⁺-chelating agent EGTA on Vγ9Vδ2 T-cell cytotoxicity toward Daudi, OCI-Ly8, and Jurkat cells in a 4-hr ⁵¹chromium release assay. As a negative control, EGTA effect was also tested on Jurkat cells lysis induced by Fas agonist CH11 mAb in the same conditions. Isotype-matched IgM antibody induced no lysis of Jurkat cells (not shown). EGTA concentrations are shown in insert box. (E/T = 20/1).