Fig. 5

Amplification of Vγ9Vδ2 T cells with the synthetic phosphoantigen PHD. Amplification of Vγ9Vδ2 T cells among PBMC cultures from four different healthy donors after 12 days of incubation with various concentrations of the synthetic phosphoantigenic Vγ9Vδ2-specific drug PHD, in the presence of IL-2, was monitored by FACS. (A) Increase in number of Vγ9Vδ2 T cells after stimulation of PBMC from four donors with three concentrations of PHD and IL-2 alone. The data give the fold increase calculated as follows: [% S2⁺CD3⁺ cells after stimulation (AS) × total viable cell number AS]/[% S2⁺CD3⁺ cells before stimulation (BS) × total viable cell number BS]. (B) Dose-dependent increase of Vγ9Vδ2 T cells from PBMC of donor 1 after PHD stimulation. Data show the dot-plots of double-staining FACS analysis from the specified culture conditions. (C) Maximal increase of Vγ9Vδ2 T cells from PBMC of the three other donors after PHD stimulation. Data show the dot-plots of double-staining FACS analysis from the specified culture conditions.