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Table 2 Amplification of V γ 9V δ 2 T cells after incubation with various NHL B-cell lymphoma

From: Synthetic Phosphoantigens Enhance Human Vγ9Vδ2 T Lymphocytes Killing of Non-Hodgkin’s B Lymphoma

Cell Lines V γ 9V δ 2 CTL Expansion from PBMC/Number of Donors Tested
DAUDI 3/3
OCI-Ly8 0/3
L428 2/3
DG75 0/2
VAL 0/3
RPMI8226 3/3
BL9 0/2
HLY-1 0/2
PASC 0/2
DEAU 2/3
NCI-H 929 0/2
RL 0/3
LIB 0/2
REC1 0/2
MIEUL 0/2
RAJI 0/3
  1. After 13-day co-culture of primary PBMC from healthy donors with irradiated NHL-cells, viable CD3+TCR-Vδ2+ cells were quantified by FACS. Amplification was considered negative when percentage of CD3+TCRVδ2+ cells in co-cultures was similar to that of PBMC cultured with IL-2 only. For each NHL, data summarize the ratio of amplification-reactive donors versus number of donors tested. For example, with the stimulating NHL DEAU, representative percentages of CD3+TCRVδ2+ cells are: donor 1, 9.9% in co-culture versus 3.1% for IL-2 alone; donor 2, 3.8% in co-culture versus 2.4% for IL-2 alone; donor 3, 18.1% in co-culture versus 2.8% for IL-2 alone. With nonstimulating NHL DG75: representative percentages of CD3+TCRVδ2+ cells are donor 1, 2.1% in co-culture versus 3.1% for IL-2 alone; donor 2, 1.1% in co-culture versus 2.4% for IL-2 alone.