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Table 2 Amplification of V γ 9V δ 2 T cells after incubation with various NHL B-cell lymphoma

From: Synthetic Phosphoantigens Enhance Human Vγ9Vδ2 T Lymphocytes Killing of Non-Hodgkin’s B Lymphoma

Cell Lines

V γ 9V δ 2 CTL Expansion from PBMC/Number of Donors Tested

DAUDI

3/3

OCI-Ly8

0/3

L428

2/3

DG75

0/2

VAL

0/3

RPMI8226

3/3

BL9

0/2

HLY-1

0/2

PASC

0/2

DEAU

2/3

NCI-H 929

0/2

RL

0/3

LIB

0/2

REC1

0/2

MIEUL

0/2

RAJI

0/3

  1. After 13-day co-culture of primary PBMC from healthy donors with irradiated NHL-cells, viable CD3+TCR-Vδ2+ cells were quantified by FACS. Amplification was considered negative when percentage of CD3+TCRVδ2+ cells in co-cultures was similar to that of PBMC cultured with IL-2 only. For each NHL, data summarize the ratio of amplification-reactive donors versus number of donors tested. For example, with the stimulating NHL DEAU, representative percentages of CD3+TCRVδ2+ cells are: donor 1, 9.9% in co-culture versus 3.1% for IL-2 alone; donor 2, 3.8% in co-culture versus 2.4% for IL-2 alone; donor 3, 18.1% in co-culture versus 2.8% for IL-2 alone. With nonstimulating NHL DG75: representative percentages of CD3+TCRVδ2+ cells are donor 1, 2.1% in co-culture versus 3.1% for IL-2 alone; donor 2, 1.1% in co-culture versus 2.4% for IL-2 alone.