Fig. 6
From: Systemic p53 Gene Therapy of Cancer with Immunolipoplexes Targeted by Anti-Transferrin Receptor scFv
TfRscFv-immunolipoplex-mediated gene transfection in vitro, using another reporter gene, the firefly luciferase gene. (a) Human breast cancer cell line, MDA-MB-435. (b) Human prostate cancer cell line, DU145. Transfection efficiency was assessed by the Luciferase assay and is given as relative light units(Rlu)/µg protein, as previously described (16). The TfRscFv significantly increased the transfection efficiency of cationic liposomes. TfRscFv-LipA2-pLuc was 10-fold more efficient than non-targeted LipA-pLuc and 2- to 3-fold more efficient than Tf-LipA-pLuc. Control scFv (CTLscFv)-LipA-pLuc showed the same activity as LipA-pLuc.