Fig. 3
Lysozyme suppresses AGE-mediated macrophage gene induction.
Confluent cultures of murine macrophagelike T1B-183 cells (1 × 106/well) were incubated with either AGE-BSA (200 µg/ml) or, BSA (200 µg/ml), in the presence or absence of LZ (100 µg/ml), which was added with or without anti-LZ antiserum (50 µg/ml) for up to 24 hrs at 37°C. Total RNA was extracted for TNFα (A,B), and IGF-I (E,F) mRNA assessment by RT-PCR. (C,D) MG-BSA (25 µg/ml) was added to T1B-183 cells with/without LZ (100 µg/ml) and RNA was extracted for TNFα mRNA testing by RT-PCR. β-actin is shown as an internal control gene. Data are representative of at least three identical experiments, each done in duplicate.