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Table 2 LZ suppresses AGE-induced mesangial cell expression of growth promoting genes in culture §

From: Lysozyme Enhances Renal Excretion of Advanced Glycation Endproducts In Vivo and Suppresses Adverse AGE-mediated Cellular Effects In Vitro: A Potential AGE Sequestration Therapy for Diabetic Nephropathy?

mRNA *

Control

LZ

AGE

AGE + LZ

PDGF-B/β-actin(A.U.)

0.12 ± 0.05

0.164 ± 0.04

0.49 ± 0.11**

0.27 ± 0.07#

TGF-β 1/β-actin (A.U.)

0.21 ± 0.04

0.19 ± 0.08

0.24 ± 0.09

0.26 ± 0.03

α1 (IV)/β-actin (A.U.)

0.27 ± 0.07

0.29 ± 0.13

0.74 ± 0.12**

0.39 ± 0.06#

Tenascin/β-actin (A.U.)

0.05 ± 0.01

0.04 ± 0.02

0.19 ± 0.06**

0.07 ± 0.02##

  1. **p < 0.05, vs Control. #p < 0.05, ##p < 0.01, vs AGE.
  2. *Data are expressed as the ratio of each faxtor cDNA/mutant to β-actin cDNA/mutant and are shown as the M ± SD of three experiments, each performed in duplicate.
  3. §Primary murine mesangial cell cultures (1 × 105/well) were incubated with AGE-BSA (800 µg/ml), AGE-BSA (800 µg/ml) + LZ (400 µg/ml) or media (DMEM/F12 + 20% FCS) (Control) or LZ alone (400 µg/ml) for up to 3 d. mRNA expression for PDGF-B, α1 type IV collagen, or tenascin was determined by RT-PCR.