mRNA
*
|
Control
|
LZ
|
AGE
|
AGE + LZ
|
---|
PDGF-B/β-actin(A.U.) | 0.12 ± 0.05 | 0.164 ± 0.04 | 0.49 ± 0.11** | 0.27 ± 0.07# |
TGF-β 1/β-actin (A.U.) | 0.21 ± 0.04 | 0.19 ± 0.08 | 0.24 ± 0.09 | 0.26 ± 0.03 |
α1 (IV)/β-actin (A.U.) | 0.27 ± 0.07 | 0.29 ± 0.13 | 0.74 ± 0.12** | 0.39 ± 0.06# |
Tenascin/β-actin (A.U.) | 0.05 ± 0.01 | 0.04 ± 0.02 | 0.19 ± 0.06** | 0.07 ± 0.02## |
- **p < 0.05, vs Control. #p < 0.05, ##p < 0.01, vs AGE.
- *Data are expressed as the ratio of each faxtor cDNA/mutant to β-actin cDNA/mutant and are shown as the M ± SD of three experiments, each performed in duplicate.
- §Primary murine mesangial cell cultures (1 × 105/well) were incubated with AGE-BSA (800 µg/ml), AGE-BSA (800 µg/ml) + LZ (400 µg/ml) or media (DMEM/F12 + 20% FCS) (Control) or LZ alone (400 µg/ml) for up to 3 d. mRNA expression for PDGF-B, α1 type IV collagen, or tenascin was determined by RT-PCR.